The effect of picric acid on the aggregation of human erythrocytes was studied. The addition of picric acid to a suspension of washed erythrocytes was shown to result in a decrease in medium pH to 1.5–2 and the formation of echinocytes. Stirring a suspension of echinocytes at low pH values caused an enhanced aggregation of cells. An increase in medium pH to 7.4 led to the dissolution of echinocyte aggregates. It was found that picric acid did not induce the aggregation of cells fixed with glutaraldehyde. The aggregation of spherical erythrocytes obtained after warming the cells at 50°C decreases considerably.

References

1. D. Danon, Bibl. Anat. 11, 289–295 (1973).
2. K. M. Jan and S. Chien, J. Gen. Physiol. 61, 638–654 (1973).
3. W. T. Coakley, L. A. Hewison, and D. Tilley, Eur. J. Biophys. 13, 123–130 (1985).
4. S. M. Gokhale and M. G. Medha, Biochem. J. 241, 505–511 (1987).
5. W. T. Coakley and D. Gallez, Biosci. Rep. 9, 675–691 (1989).
6. D. Gallez and W. T. Coakley, Heterogeneous Chem. Rev. 3, 443–475 (1996).
7. M. Rolfe, A. Parmar, T. G. Hog, and W. T. Coakley, Mol. Membr. Biol. 18, 169–176 (2001).
8. Yu. A. Sheremet'ev, F. Yu. Suslov, A. V. Deryugina, and A. V. Sheremet'eva, Biofizika 45 (1), 79–82 (2000).
9. Yu. A. Sheremet'ev, F. Yu. Suslov, and A. V. Sheremet'eva, Tsitologiya 43, 648–651 (2001).
10. D. Lerche and R. Glaser, Acta Biol. Med. Germ. 39, 973–978 (1980).
11. D. Lerche, Biorheology 19, 587–598 (1982).
12. E. Hessel and D. Lerche, Vox. Sang 49, 86–91 (1985).
13. M. P. Sheetz and S. J. Singer, J. Cell Biol. 70, 247–251 (1976).
14. M. P. Sheetz, R. G. Painter, and S. J. Singer, J. Cell Biol. 70, 193–203 (1976).
15. H. J. Meselman, Biorheology 15, 225–238 (1978).
16. S. R. P. Gudi, A. Kumar, V. Bhakuni, et al., Biochim. Biophys. Acta 1023, 63–72 (1990).
17. Yu. A. Sheremet'ev and F. Yu. Suslov, Gematol. Transfuziol. 38, 35–36 (1993).
18. Yu. A. Sheremet'ev and F. Yu. Susov, RF Patent No. 2 082 968;
    Byull. Izobret., No. 18, p. 192 (1997).